货号: 21006 |
基因符号: Gα i |
描述: Anti-Gα i Rabbit Polyclonal Antibody |
背景介绍: Heterotrimeric G proteins are critical cellular ** transducers. Gα irepresents one sub-family of G proteins that could mediate the inhibition of adenylyl cyclases. Other biochemical and physiological functions of Gα i proteins are being explored. |
*原: Recombinant full length protein of Gα i1 |
经过测试的应用: ELISA, WB, IHC |
推荐稀释比: ELISA 1: 100-1: 5000 WB 1: 100-1: 2000 |
浓度: 1 mg/ml |
宿主: Rabbit |
形式: Liquid |
克隆性: Polyclonal |
纯度: Purified from serum |
防腐剂: No |
成分: PBS (without Mg 2+ and Ca 2+ ), pH 7.4, 150 mM NaCl, 50% glycerol |
种属反应性: Gα i antibody recognizes Gα i1, Gα i2, and Gα i3 of vertebrates |
储存条件: Store at -20°C. Avoid repeated freezing and thawing |
蛋白质印迹: Western blot analysis of Gi proteins. Gα i1 plasmid DNA was transiently transfected into HEK-293 cells. Whole-cell lysate was used for western blot with the polyclonal anti Gα i antibody (Cat. # 21006). |
1.Bioinformatic analysis of membrane and associated proteins in murine cardiomyocytes and human myocardium Sci Data. 2020 PMID: 33262348 |
2.A G protein-coupled a7 nicotinic receptor regulates **ing and TNF-a release in microglia FEBS Open Bio. 2017 PMID: 28904864 |
3.Fatty Acid-binding Protein 5 (FABP5) Regulates Cognitive Function Both by Decreasing Anandamide Levels and by Activating the Nuclear Receptor Peroxisome Proliferator-activated Receptor β/δ (PPARβ/δ) in the Brain J Biol Chem. 2014 PMID: 24644281 |
4.Identification of novel **ling roles and targets for Galpha and Gbetagamma downstream of the insulin-like growth factor 1 receptor in vascular smooth muscle cells Biochem J. 2013 PMID: 23186281 |
5.Muscarinic acetylcholine receptor-mediated activation of G(q) in rat brain membranes determined by nosine-5′-O-(3-[35S]thio)triphosphate ([35S]GTPγS) binding using an anti-G protein scintillation proximity assay J Neural Transm (Vienna). 2012 PMID: 22127550 |
6.Na/H exchanger regulatory factors control parathyroid hormone receptor **ing by facilitating differential activation of G(alpha) protein subunits J Biol Chem. 2010 PMID: 20562104 |