货号: 26191 |
基因符号: H-Ras; K-Ras; N-Ras |
描述: Anti-HRAS(G13A) Mouse Monoclonal Antibody |
背景介绍: Belongs to the small GTPase superfamily. Ras family binds GDP/GTP and possess intrinsic GTPase activity, alternate between an inactive form bound to GDP and an active form bound to GTP. Activated by a nine nucleotide-exchange factor (GEF) and inactivated by a GTPase-activating protein ().Located in cell membrane, lipid-anchor, cytoplasmic side and Golgi apparatus membrane. |
*原: A synthetic peptide from the internal region of HRas which includes the mutation of G13A, human origin |
经过测试的应用: ELISA, WB, IHC |
推荐稀释比: ELISA: 1: 1000-1: 5000 WB: 1: 500-1: 1000 IHC: 1: 50-1: 100 |
浓度: 1 mg/ml |
宿主: Mouse |
形式: Liquid |
克隆性: Monoclonal |
亚型: IgG |
纯度: Purified from ascites |
防腐剂: No |
成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
种属反应性: Recognizes G13A mutant, but not wild-type H-RAS of vertebrates |
储存条件: Store at -20°C. Avoid repeated freezing and thawing |
蛋白质印迹: Western blot analysis of recombinant HRAS(G13A) and wildtype proteins. Purified His-tagged HRAS(G13A) protein (lane 2) and corresponding wildtype protein (lane 1) were blotted with Anti-HRAS(G13A) monoclonal antibody (Cat. #26191). |