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武汉费斯德生物科技有限公司
主营：G蛋白活性抗体及试剂盒,非乙酰化CAMP和CGMP试剂,点突变抗体 - 18162422833
武汉费斯德生物科技有限公司
主营：G蛋白活性抗体及试剂盒,非乙酰化CAMP和CGMP试剂,点突变抗体
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| 货号: 26347 |
| 基因符号: FBW6; FBW7; FBXW7; FBX30; CDC4; SEL10 |
| 描述: Anti-FBXW7(R479L) Mouse Monoclonal Antibody |
| 背景介绍: F-box/WD repeat-containing protein 7 is a protein that in humans is encoded by the FBXW7 gene. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. Mutations in this gene are detected in ovarian, breast and colorectal cancer cell lines, implicating the gene’s potential role in the pathogenesis of human cancers. |
| *原: A synthetic peptide from the internal region of FBXW7, which includes the mutation of R479L, human origin |
| 经过测试的应用: ELISA, WB |
| 推荐稀释比: ELISA: 1: 1000-1: 5000 WB: 1: 500-1: 1000 |
| 浓度: 1 mg/ml |
| 宿主: Mouse |
| 形式: Liquid |
| 克隆性: Monoclonal |
| 亚型: IgG |
| 纯度: Purified from ascites |
| 防腐剂: No |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| 种属反应性: Recognizes R479L mutant, but not wild-type FBXW7 of vertebrates |
| 储存条件: Store at -20°C. Avoid repeated freezing and thawing |
蛋白质印迹: Western blot analysis of recombinant FBXW7(R479L) and wildtype proteins. Purified His-tagged FBXW7(R479L) protein (lane 2) and corresponding wildtype protein (lane 1) were blotted with Anti-FBXW7(R479L) monoclonal antibody (Cat. #26347). Immunofluorescence of cells expressing FBXW7 proteins with Anti-FBXW7(R479L) antibody. HEK293T cells were transfected with p3-GFP-FBXW7 (WT) plasmid (left column) or p3-GFP-FBXW7(R479L) plasmid (right column), then fixed and stained with Anti-FBXW7(R479L) monoclonal antibody (Cat. #26347). |