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武汉费斯德生物科技有限公司
主营：G蛋白活性抗体及试剂盒,非乙酰化CAMP和CGMP试剂,点突变抗体 - 18162422833
武汉费斯德生物科技有限公司
主营：G蛋白活性抗体及试剂盒,非乙酰化CAMP和CGMP试剂,点突变抗体
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| 货号: 26287 |
| 基因符号: EGFR/HER1/ERBB1 |
| 描述: Anti-EGFR(L747-P753)S Mouse Monoclonal Antibody |
| 背景介绍: EGFR is a receptor tyrosine kinase binding ligands of the EGF family and activating several **ing cascades to convert extracellular cues into appropriate cellular responses. It activates at least 4 major downstream **ing cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. Defects in EGFR are associated with lung cancer (LNCR). |
| *原: A synthetic peptide from the internal region of EGFR, which includes the mutation of (L747-P753) S, human origin |
| 经过测试的应用: ELISA, WB, IF, IHC |
| 推荐稀释比: ELISA: 1: 1000-1: 5000 WB: 1: 500-1: 1000 IHC: 1: 50-1: 100 |
| 浓度: 1 mg/ml |
| 宿主: Mouse |
| 形式: Liquid |
| 克隆性: Monoclonal |
| 亚型: IgG |
| 纯度: Purified from ascites |
| 防腐剂: No |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| 种属反应性: Recognizes EGFR(L747-P753)S, but not wild type EGFR protein from vertebrates |
| 储存条件: Store at -20°C. Avoid repeated freezing and thawing |
蛋白质印迹:
Western blot analysis of recombinant EGFR(L747-P753) S and wild type proteins.
Purified His-tagged EGFR(L747-P753)S protein (702-987) and wild-type protein (702-987) were blotted with anti- EGFR(L747-P753)S mouse antibody (Cat. # 26287).
*荧光:
Immunofluorescence of cells expressing EGFR proteins with anti-EGFR(L747-P753)S antibody.
HEK293T cells were transfected with p3-GFP-EGFR (WT) plasmid (left column) or p3-GFP-EGFR(L747-P753)S plasmid (right column), then fixed and stained with anti- EGFR(L747-P753)S monoclonal antibody (Cat. #26287).