-
武汉费斯德生物科技有限公司
主营：G蛋白活性抗体及试剂盒,非乙酰化CAMP和CGMP试剂,点突变抗体 - 18162422833
武汉费斯德生物科技有限公司
主营：G蛋白活性抗体及试剂盒,非乙酰化CAMP和CGMP试剂,点突变抗体
4
| 货号: 26302 |
| 基因符号: Beta-catenin, CTNNB, Catenin beta-1 |
| 描述: Anti-CTNNB1(D32Y) Mouse Monoclonal Antibody |
| 背景介绍: CTNNB1 protein is a dual function protein. It is a subunit of a complex of proteins that from adherent junctions, which are important for the establishment and maintenance of epithelial cell layers by regulating cell growth and adhesion between adjacent cells. CTNNB1 protein also pulls double duty as an intracellular ** transducer in the Wnt **ing pathway. Mutations of CTNNB1 have been implicated in the pathogenesis of several cancers. |
| *原: A synthetic peptide from the internal region of CTNNB1, which includes the mutation of D32Y, human origin |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: ELISA: 1: 1000-1: 2000 WB: 1: 500-1: 1000 IHC: 1: 50-1: 100 |
| 浓度: 0.5 mg/ml |
| 宿主: Mouse |
| 形式: Liquid |
| 克隆性: Monoclonal |
| 亚型: IgG |
| 纯度: Purified from ascites |
| 防腐剂: No |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| 种属反应性: Recognizes D32Y mutant, but not wild-type CTNNB1 of vertebrates |
| 储存条件: Store at -20°C. Avoid repeated freezing and thawing |
蛋白质印迹: Purified His-tagged CTNNB1(D32Y) protein (lane2) and corresponding wild-type protein (lane1) were blotted with anti-CTNNB1(D32Y) monoclonal antibody (Cat. #26302). HEK293T cells were transfected with p3-GFP-CTNNB1(D32Y) plasmid, p3-GFP-CTNNB1 (WT) plasmid or p3-GFP-CTNNB1 (D32N) plasmid, then fixed and stained with anti-CTNNB1(D32Y) monoclonal antibody (Cat. #26302). |