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武汉费斯德生物科技有限公司
主营：G蛋白活性抗体及试剂盒,非乙酰化CAMP和CGMP试剂,点突变抗体 - 18162422833
武汉费斯德生物科技有限公司
主营：G蛋白活性抗体及试剂盒,非乙酰化CAMP和CGMP试剂,点突变抗体
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| 货号: 26406 |
| 基因符号: IDH2; D2HGA2; ICD-M; IDH; IDHM; IDP; IDPM; mNADP-IDH |
| 描述: Anti-IDH1(2P) Mouse Monoclonal Antibody |
| 背景介绍: Isocitrate dehydrogenases (IDH) catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. Mutations affecting Arg-132 are tissue-specific, and suggest that this residue plays a unique role in the development of high-grade gliomas. Mutations of Arg-132 to Cys, His, Leu or Ser abolish magnesium binding and abolish the conversion of isocitrate to alpha-ketoglutarate. Instead, alpha-ketoglutarate is converted to R-2-hydroxyglutarate. Elevated levels of R-2-hydroxyglutarate are correlated with an elevated risk of malignant brain tumors. |
| *原: A synthetic peptide from the internal region of IDH1 which includes the mutation of 2P, human origin |
| 经过测试的应用: ELISA, WB, IF, IHC |
| 推荐稀释比: ELISA: 1: 1000-1: 5000 WB: 1: 100-1: 1000 IF: 1: 50-1: 100 IHC: 1: 50-1: 100 |
| 浓度: 1 mg/ml |
| 宿主: Mouse |
| 形式: Liquid |
| 克隆性: Monoclonal |
| 亚型: IgG |
| 纯度: Purified from ascites |
| 防腐剂: No |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| 种属反应性: recognizes 2P mutant, but not wild-type IDH2 of vertebrates |
| 储存条件: Store at -20°C. Avoid repeated freezing and thawing |
| 蛋白质印迹: Western blot analysis of recombinant IDH1(2P) and wildtype proteins. Purified His-tagged IDH1(2P) (lane 1) and corresponding wild-type IDH1 protein (lane 2) were blotted with Anti-IDH1(2P) monoclonal antibody (Cat. #26406). *荧光: HEK293T cells were transfected with p3-GFP-IDH1(2P) plasmid, p3-GFP-IDH1 (WT) plasmid , p3-GFP-IDH1(2S) plasmid, p3-GFP-IDH1(2L) plasmid , p3-GFP-IDH1(2G) plasmid or p3-GFP-IDH1(2C) plasmid , then fixed and stained with Anti-IDH1(2P) monoclonal antibody (Cat. #26406). |