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武汉费斯德生物科技有限公司
主营：G蛋白活性抗体及试剂盒,非乙酰化CAMP和CGMP试剂,点突变抗体 - 18162422833
武汉费斯德生物科技有限公司
主营：G蛋白活性抗体及试剂盒,非乙酰化CAMP和CGMP试剂,点突变抗体
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| 货号: 26039 |
| 基因符号: BRaf |
| 描述: Anti-BRaf(V600E) Mouse Monoclonal Antibody |
| 背景介绍: B-Raf is a member of the Raf family of Ser/Thr protein kinases. It functions downstream of Ras to regulate the MAP kinase **ing pathway. Mutations in the BRAF gene cause diseases. Inherited mutations in BRAF cause cardiofaciocutaneous syndrome. Acquired mutations in BRAF have been found in cancers |
| *原: A synthetic peptide from the internal region of BRaf(V600E), human origin |
| 经过测试的应用: ELISA, WB, IHC, IP |
| 推荐稀释比: ELISA: 1: 100-1: 5000 WB: 1: 100-1: 2000 IHC: 1: 100-1: 1000 |
| 浓度: 1 mg/ml |
| 宿主: Mouse |
| 形式: Liquid |
| 克隆性: Monoclonal |
| 亚型: IgG2b |
| 纯度: Purified from ascites |
| 防腐剂: No |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| 种属反应性: Recognizes B-Raf(V600E), but not wild type B-Raf proteins from vertebrates. |
| 储存条件: Store at -20°C. Avoid repeated freezing and thawing |
蛋白质印迹:
Western blot analysis of recombinant B-raf proteins with anti-BRaf(V600E) antibody.
Purified His – tagged B-Raf( V600E ) ?amino acids 513-693)(lane1) ? corresponding wild type protein(lane 2), His – tagged B-Raf( V600D ) (lane 3) , His – tagged B-Raf( V600A ) (lane 4) , His – tagged B-Raf( V600G ) (lane 5) , His – tagged B-Raf( V600K ) (lane 6), His – tagged B-Raf( V600R ) (lane 7), and His – tagged B-Raf( V600M ) (lane 8) were blotted with anti-B-Raf(V600E) monoclonal antibody (Cat. # 26039) .
*荧光:
Immunofluorescence of cell expressing BRaf protein with anti-BRaf(V600E) antibody . HEK293T cells were transfected with p3-GFP- B-Raf( V600E )plasmid,p3-GFP- B-Raf( WT )plasmid, p3-GFP- B-Raf( V600K )plasmid,p3-GFP- B-Raf( V600R )plasmid, p3-GFP- B-Raf( V600A )plasmid,p3-GFP- B-Raf( V600D ) plasmid, p3-GFP-B-Raf(V600M)plasmid, or p3-GFP-B-Raf(V600G)plasmid, then fixed and stained with anti-B-Raf(V600E) monoclonal antibody (Cat. # 26039).
Immunohistochemistry:
B-Raf(V600E) monoclonal antibody (Cat. # 26039). Tissue samples were fixed with formaldehyde and blocked with 1% serum for 15 min at 37 °C. Antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were then incubated with primary antibody (1: 100) overnight at 4°C. A HRP-conjugated Goat anti-mouse IgG (dilution 1: 50) was used as secondary antibody.
Immunohistochemical analysis of paraffin-embedded Thyroid Carcinoma tissue -with anti-BRaf (V600E) monoclonal antibody (Cat. # 26039). Tissue samples were fixed with paraffin. Samples were then incubated with primary antibody (1: 100) overnight at 4°C. An HRP-conjugated Goat anti-mouse IgG (dilution 1: 50) was used as the secondary antibody. Allele-specific PCR validated to be Negative for BRAF V600E.
Immunohistochemical analysis of paraffin-embedded Thyroid Carcinoma tissue -with anti BRaf(V600E) monoclonal antibody (Cat. # 26039). Tissue samples were fixed with paraffin. Samples were then incubated with primary antibody (1: 100) overnight at 4°C.A HRP-conjugated Goat anti-mouse IgG (dilution 1: 50) was used as secondary antibody.Allele specific PCR validated to be Negative for BRAF V600E.