-
武汉费斯德生物科技有限公司
主营：G蛋白活性抗体及试剂盒,非乙酰化CAMP和CGMP试剂,点突变抗体 - 18162422833
武汉费斯德生物科技有限公司
主营：G蛋白活性抗体及试剂盒,非乙酰化CAMP和CGMP试剂,点突变抗体
3
| 货号: 26199 |
| 基因符号: BRAF, BRAF1, RAFB1 |
| 描述: Anti-BRAF(V600A) Mouse Monoclonal Antibody |
| 背景介绍: BRAF belongs to a family of serine-threonine protein kinases. As a part of a **ing pathway known as the the RAS/MAPK pathway, it plays an important part in many cellular processes. The processes include cell proliferation, differentiation and transcriptional regulation. Mutations in the BRAF gene cause diseases. Inherited mutations in BRAF cause cardiofaciocutaneous syndrome. Acquired mutations in BRAF have been found in cancers. |
| *原: A synthetic peptide from the internal region of BRAF, which includes the mutation of V600A, human origin |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: ELISA: 1: 1000-1: 5000 WB: 1: 500-1: 1000 IF: 1: 50-1: 100 IHC: 1: 25-1: 100 |
| 浓度: 1 mg/ml |
| 宿主: Mouse |
| 形式: Liquid |
| 克隆性: Monoclonal |
| 亚型: IgG |
| 纯度: Purified from ascites |
| 防腐剂: No |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| 种属反应性: Recognizes V600A mutant, but not wild-type BRAF of vertebrates |
| 储存条件: Store at -20°C. Avoid repeated freezing and thawing |
| 蛋白质印迹: Western blot analysis of recombinant BRAF proteins. Purified His-tagged BRAF(V600A) protein (lane 1) , corresponding wildtype protein (lane 2) , His-tagged BRAF (V600R) protein (lane 3), His-tagged BRAF (V600M) protein (lane 4), His-tagged BRAF (V600K) protein (lane 5), His-tagged BRAF (V600G) protein (lane 6), His-tagged BRAF (V600D) protein (lane 7) and His-tagged BRAF (V600E) protein (lane 8) were blotted with Anti-BRAF(V600A) monoclonal antibody (Cat. #26199). Immunofluorescence of cells expressing BRAF proteins with Anti-BRAF(V600A) antibody. HEK293T cells were transfected with p3-GFP-BRAF (WT) plasmid, p3-GFP-BRAF(V600A) plasmid, p3-GFP-BRAF (V600D) plasmid, p3-GFP-BRAF (V600G) plasmid p3-GFP-BRAF (V600M) plasmid or p3-GFP-BRAF (V600E) plasmid, then fixed and stained with Anti-BRAF(V600A) monoclonal antibody (Cat. #26199). |