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武汉费斯德生物科技有限公司
主营：G蛋白活性抗体及试剂盒,非乙酰化CAMP和CGMP试剂,点突变抗体 - 18162422833
武汉费斯德生物科技有限公司
主营：G蛋白活性抗体及试剂盒,非乙酰化CAMP和CGMP试剂,点突变抗体
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| 货号: 26109 |
| 基因符号: BRAF, BRAF1, RAFB1 |
| 描述: Anti-BRaf(N581S) Mouse Monoclonal Antibody |
| 背景介绍: B-Raf is a member of the Raf family of Ser/Thr protein kinases. It functions downstream of Ras to regulate the MAP kinase **ing pathway. Mutations in the BRAF gene cause diseases. Inherited mutations in BRAF cause cardiofaciocutaneous syndrome. Acquired mutations in BRAF have been found in cancers. |
| *原: A synthetic peptide from the internal region of Braf (N581S), human origin |
| 经过测试的应用: ELISA, WB, IHC, IP |
| 推荐稀释比: ELISA: 1: 1000-1: 5000 WB: 1: 500-1: 2000 IHC: 1: 100-200 |
| 浓度: 0.5 mg/ml |
| 宿主: Mouse |
| 形式: Liquid |
| 克隆性: Monoclonal |
| 亚型: IgG |
| 纯度: Purified from ascites |
| 防腐剂: No |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| 种属反应性: Recognizes B-Raf N581S, but not wild-type B-Raf proteins from vertebrates |
| 储存条件: Store at -20°C. Avoid repeated freezing and thawing |
蛋白质印迹: Western blot analysis of recombinant B-raf proteins. Purified His-tagged BRaf(N581S) protein (amino acids 513-693, lane 2) and corresponding wild type protein (lane 1) were blotted with Anti-BRaf(N581S) monoclonal antibody (Cat. # 26109). *荧光: Immunofluorescence of cells expressing B-Raf proteins with Anti-BRaf(N581S) antibody. HEK293T cells were transfected with p3-GFP-B-Raf (WT) plasmid (left column) or p3-GFP-BRaf(N581S) plasmid (right column), then fixed and stained with Anti-BRaf(N581S) monoclonal antibody (Cat. #26109). |