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武汉费斯德生物科技有限公司
主营：G蛋白活性抗体及试剂盒,非乙酰化CAMP和CGMP试剂,点突变抗体 - 18162422833
武汉费斯德生物科技有限公司
主营：G蛋白活性抗体及试剂盒,非乙酰化CAMP和CGMP试剂,点突变抗体
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** 本公司销售的所有产品仅供实验科研使用，不用于人体及临床诊断。
Anti-ABL(Y253H) Mouse Monoclonal Antibody
| 背景介绍: V-abl Abelson murine leukemia viral oncogene homolog 1 also known as ABL1, is a cytoplasmic and nuclear protein tyrosine kinase that has been implicated in processes of cell differentiation, cell division, cell adhesion, stress response and cell migration. Deficient in Abl can cause severe disease such as leukemia. |
| *原: A synthetic peptide from the internal region of Abl which includes the mutation of Y253H, human origin |
| 经过测试的应用: ELISA, WB, IHC, IF |
| 推荐稀释比: ELISA: 1: 1000-1: 5000 WB: 1: 100-1: 1000 IF: 1: 50-1: 100 IHC: 1: 50-1: 100 |
| 浓度: 1 mg/ml |
| 宿主: Mouse |
| 形式: Liquid |
| 克隆性: Monoclonal |
| 亚型: IgG |
| 纯度: Purified from ascites |
| 防腐剂: No |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| 种属反应性: recognizes Abl Y253H, but not wildtype of vertebrates |
| 储存条件: Store at -20°C. Avoid repeated freezing and thawing |
| 蛋白质印迹: Western blot analysis of recombinant Abl(Y253H) and wildtype proteins. Purified His-tagged Abl(Y253H) protein (amino acids 1-76, lane 2) and corresponding wild type protein (lane 1) were blotted with anti-ABL(Y253H) mouse monoclonal antibody (Cat. #26154). |
| *荧光: Immunofluorescence of cells expressing Abl proteins with anti-Abl(Y253H) antibody. HEK293T cells were transfected with pCDNA3-GFP-Abl (WT) plasmid (left column) or pCDNA3-GFP-Abl(Y253H) plasmid (right column), then fixed and stained with anti-ABL(Y253H) monoclonal antibody (Cat. #26154). |