货号:26918
背景介绍: ARF6 (ADP-ribosylation factor 6) is a member of the ARF super-family. ARF genes encode small GTPases that increase the ADP-ribosyltransferase activity of cholera toxin and are critical for vesicular trafficking as activators of phospholipase D. Arf6 regulates membrane trafficking and functions as a regulatory molecule of phagocytosis.
*原: Recombinant full length protein of active Arf6.
经过测试的应用: IP, IHC, IF
推荐稀释比:
IP: 1 µg for 1~2 mg total cellular proteins
IHC, IF: 1:50-1:250
*沉淀/蛋白质印迹: |
Arf6 activation assay. Purified full-length Arf6 proteins were immunoprecipitated after treated with GDP (lane left) or GTPγS (lane right). Immunoprecipitation was done with the anti-Arf6-GTP monoclonal antibody (Cat. No. 26918). Immunoblot was with an anti-Arf6 polyclonal antibody (Cat. No. 21032). |