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武汉费斯德生物科技有限公司
主营：G蛋白活性抗体及试剂盒,非乙酰化CAMP和CGMP试剂,点突变抗体 - 18162422833
武汉费斯德生物科技有限公司
主营：G蛋白活性抗体及试剂盒,非乙酰化CAMP和CGMP试剂,点突变抗体
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| 货号: 26182 |
| 基因符号: BRAF, BRAF1, RAFB1 |
| 描述: Anti-BRaf(V600R) Mouse Monoclonal Antibody |
| 背景介绍: B-Raf is a member of the Raf family of Ser/Thr protein kinases. It functions downstream of Ras to regulate the MAP kinase **ing pathway. Mutations in the BRAF gene cause diseases. Inherited mutations in BRAF cause cardiofaciocutaneous syndrome. Acquired mutations in BRAF have been found in cancers. |
| *原: A synthetic peptide from the internal region of BRaf(V600R), human origin |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: ELISA 1: 1000-1: 5000 WB 1: 500-1: 1500 IHC 1: 100-1: 200 |
| 浓度: 0.9 mg/ml |
| 宿主: mouse |
| 形式: Liquid |
| 克隆性: Monoclonal |
| 亚型: IgG |
| 纯度: Purified from ascites |
| 防腐剂: No |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| 种属反应性: Recognizes B-Raf V600R, but not wild-type B-Raf proteins |
| 储存条件: Store at -20°C. Avoid repeated freezing and thawing |
蛋白质印迹: Western blot analysis of recombinant B-raf proteins. Purified His-tagged BRaf(V600R) protein ( lane 2) and corresponding wild type protein (lane 1) were blotted with Anti-BRaf (V600R) monoclonal antibody (Cat. # 26182). |