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活性抗体丨Rac-GTP 小鼠单抗丨**期刊文献

品牌: 美国 NewEast Biosciences
货号: 26903
抗原: Full Recombinant Active Rac
规格: 30 微升
产品全名: Anti-Rac1-GTP Monoclonal Antibody
应用: IP, IHC, IF
种属反应性的选择: Human, Mouse and Rat
物种/宿主: Mouse

近期文献：

001.Catalpol inhibits hepatic stellate cell activation by reducing the formation and changing the contents of hepatocyte-derived extracellular vesicles
J Cell Commun Signal. 2022 PMID: 36508052

002.Cardiac inducing colonies halt fibroblast activation and induce cardiac/endothelial cells to move and expand via paracrine **ing
Mol Biol Cell. 2022 PMID: 35653297

003.Inhibition of Rac1 attenuates radiation-induced lung injury while suppresses lung tumor in mice
Cell Death Discov. 2022 PMID: 35031595

004.Smooth muscle Rac1 contributes to pulmonary hypertension
BJP. 2022 PMID: 35064565

005.Rac1 mediates cadherin-11 induced cellular pathogenic processes in aortic valve calcification
Cardiovasc Pathol. 2022 PMID: 35074515

006.A Portable Controllable Compressive Stress Device to Monitor Human Breast Cancer Cell Protrusions at Single-Cell Resolution
J Clin Invest. 2022 PMID: 35104805

007.Hepatic Ischemia-reperfusion Injury in Mice was Alleviated by Rac1 Inhibition – More Than Just ROS-inhibition
J Clin Transl Hepatol. 2022 PMID: 35233372

008.Targeting the ASMase/S1P pathway protects from sortilin-evoked vascular damage in hypertension
J Neuroinflammation. 2022 PMID: 35284404

009.Planar cell polarity-dependent asymmetric organization of microtubules for polarized positioning of the basal body in node cells
Development. 2022 PMID: 35420656

010.Time of Day-Dependent Alteration of Hippocampal Rac1 Activation Regulates Contextual Fear Memory in Rats
Front Mol Neurosci. 2022 PMID: 35782392

011.Transient neuroinflammation following surgery contributes to long-lasting cognitive decline in elderly rats via dysfunction of synaptic NMDA receptor
Front Bioeng Biotechnol. 2022 PMID: 35831873

012.ARHGEF37 overexpression promotes extravasation and metastasis of hepatocellular carcinoma via directly activating Cdc42
J Exp Clin Cancer Res. 2022 PMID: 35869555

anoid-on-a-chip model of human ARPKD reveals mechanosensing pathomechanisms for drug discovery
Sci Adv. 2022 PMID: 36129975

014.Differential dysregulation of granule subsets in WASH-deficient neutrophil leukocytes resulting in inflammation
Nat Commun. 2022 PMID: 36130971

015.The Pro-Fibrotic Response to Lens Injury Is Signaled in a PI3K Isoform-Specific Manner
Biomolecules. 2022 PMID: 36139020

016.Anti-ANGPTL3-FLD monoclonal antibody treatment ameliorates podocyte lesions through attenuating mitochondrial damage
Cell Death Dis. 2022 PMID: 36229446

017.Critical role of mitogen-inducible gene 6 in restraining endothelial cell permeability to maintain vascular homeostasis
J Cell Commun Signal. 2022 PMID: 36284029

018.PTEN Protein Phosphatase Activity Is Not Required for Tumour Suppression in the Mouse Prostate
Biomolecules. 2022 PMID: 36291720

019.Dock4 is required for the maintenance of cochlear hair cells and hearing function

Fundamental Research. 2022

背景介绍: Small GTPases are a super-family of cellular **ing regulators. Rac belongs to the Rho sub-family of GTPases that regulate cell motility, cell division, and gene transcription. GTP binding increases the activity of Rac, and the hydrolysis of GTP to GDP renders it inactive. GTP hydrolysis is aided by GTPase activating proteins (s), while exchange of GDP for GTP is facilitated by nine nucleotide exchange factors (GEFs).

*原: Recombinant full length protein of active Rac1

经过测试的应用: IP, IHC, IF

推荐稀释比: IP: 1 µg for 1~2 mg total cellular proteins IHC, IF: 1:50-1:250

浓度: 1 mg/ml

*沉淀/蛋白质印迹: Rac Activation Assay. MEF cells were treated with (lane 2) or without (lane 1) PDGF. Cell lysates were incubated with an anti-Rac-GTP monoclonal antibody (Cat. # 26903) (top panel). Th precipitated active Rac was immunoblotted with an anti-Rac rabbit polyclonal antibody (Cat. # 21003). The bottom panel shows the Western blot with anti-Rac of the cell lysates used (5% of that used in the top panel).

*荧光: Immunofluorescence for the active Rac using Anti-Rac1-GTP Mouse Monoclonal Antibody [26903] shows Rac-GTP immunolabeling (green) in combination with cofilin (red) on brain tissue sections. The tissue sections were fixed with -20 ºC methanol or 4% paraformaldehyde (fixation time 1 hr) and stained with antibody at 1: 1000 in 0.1M Phosphate buffer with 0.3% Triton X, and 4% BSA for 24h at room temperature. Secondary antibodies were anti-mouse AlexaFluor488 and anti-rabbit AlexaFluor594 at 1: 1000.

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